Internal Ultrastructure of Human Chromosomes
نویسندگان
چکیده
منابع مشابه
Internal ultrastructure of human chromosomes.
By preparing metaphase chromosome spreads on Aclar (polychlorotrifluoroethylene fluoropolymer) film and incorporating a conventional karyotyping system, it was possible to observe the internal ultrastructure of individual chromosomes with known karyotype by means of transmission electron microscopy. Essentially, all 46 chromosomes per human cell could be examined. Application of this method to ...
متن کاملApplication of a novel technique for observing internal ultrastructure of human chromosomes with known karyotype
Observation of the internal ultrastructure of human chromosomes by transmission electron microscopy (TEM) has been hampered due to the difficulties in detaching metaphase chromosome spreads from the glass slide for further processing. We used a method in which the metaphase chromosome spreads were prepared on a flexible thermoplastic membrane, ACLAR film. To assess chromosome identity, a diamid...
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OBJECTIVES To study the ultrastructure of intraluminal defects found in the internal jugular vein by using a scanning electron microscopy. METHODS Using a scanning electron microscopy, intraluminal septa and/or defective valves blocking the flow in the distal internal jugular vein of seven patients were studied together with the adjacent wall and compared with control specimen. RESULTS The ...
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Drosophila polytene chromosomes prepared by a new micromanipulative procedure, which avoids acid squashing, have been examined at the ultrastructural level in the electron microscope. Puffs at 2B, 68C, 74EF, 75B and 85EF, have been examined in some detail, along with the chromocentre and various interbands. The ultrastructure of these chromosomes, which have never been exposed to acid protein d...
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Evidence is gradually accumulating about acquired somatic chromosome anomalies, particularly those of neoplasms. Unfortunately, control observations are almost entirely lacking. It is generally assumed that all the tissues of the body consist of cells with the normal karyotype as it is seen in cultured lymphocytes or fibroblasts, but this has not yet been shown to be the case. Even bone-marrow,...
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ژورنال
عنوان ژورنال: Journal of Nippon Medical School
سال: 2008
ISSN: 1345-4676,1347-3409
DOI: 10.1272/jnms.75.318